Jun Wang

Jun Wang
Research Professor
Bioinformatics R325

Dr. Jun Wang received his Ph.D. in Analytical Chemistry from Wuhan University, China in 2000 and was subsequently awarded a scholarship from DAAD, Germany (2000-2001). Then, he pursued his postdoc research in the California State University at Los Angeles (LA) and the University of California at LA, respectively (2001-2005). He jointed Pacific Northwest National Laboratory (PNNL) as a scientist (2005-2011). After that, Dr. Wang moved to Charlotte, NC and founded a start-up company (Nanodiagnostic Technology, LLC) in 2011 and served as the president there (2011-present).  Since October 2019, Dr. Wang became a Research Professor at the Department of Bioinformatics and Genomics at the University of North Carolina at Charlotte. Dr. Wang’s research is focused on developing nanotechnology-based assays, chemo/biosensors, and biosensor informatics for a number of applications including early detection of diseases, environmental and occupational biomonitoring, and food safety monitoring.

Lab: uncc.edu/Nanolab_Jun Wang

Selected publications:

  1. Smith, J. N.Wang, J.Lin, Y.H.Klohe, E. MTimchalk, C. Pharmacokinetics and Pharmacodynamics of Chlorpyrifos and 3,5,6-Trichloro-2-pyridinol in Rat Saliva After Chlorpyrifos Administration.  Toxicological Sciences, 2012, 130, 245-56.
  2. Du, D.; Wang, J.; Wang, L.M.; Lu, D.L.; Lin, Y.H. Integrated Lateral Flow Test Strip with Electrochemical Sensor for Quantification of Phosphorylated Cholinesterase: Biomarker of Exposure to Organophosphorus Agents. Anal. Chem. 2012, 84, 1380-1385.
  3. Shao, G. C.; Wang, J*.; Li, Z. H.; Saraf, L.; Wang, W. J.; Lin, Y. H. Poly(dimethylsiloxane) microchip-based immunoassay with multiple reaction zones: Toward on-chip multiplex detection platform. Sens. Actuat. B-Chem. 2011, 159, 44-50.
  4. Lu, D. L.; Wang, J.Wang, L.M.; Du, D.; Timchalk, C.; Barry, R.; Lin, Y.H. A Novel Nanoparticle-Based Disposable Electrochemical Immunosensor for Diagnosis of Exposure to Toxic Organophosphorus Agents. Adv. Funct. Mater. 2011, 21, 4371-4378.
  5. Du, D.; Wang, J.Lu, D. L.; Dohnalkova, A.; Lin, Y. H. Multiplexed Electrochemical Immunoassay of Phosphorylated Proteins Based on Enzyme-Functionalized Gold Nanorod Labels and Electric Field-Driven Acceleration. Anal. Chem. 2012, 83, 6580-6585.
  6. Ma-Ham, A.; Wu, H.; Wang, J.; Kang, X.H.; Zhang, Y.Y.; Lin, Y.H. Apoferritin-based nanomedicine platform for drug delivery: equilibrium binding study of daunomycin with DNA. J. Mater. Chem. 2011, 21, 8700-8708.
  7. Du, D.; Wang, J. *; Wang, L.M.; Smith, J.D.; Timchalk, C.; Lin, Y. H. Magnetic electrochemical sensing platform for biomonitoring of exposure to organophosphate pesticides and nerve agents based on simultaneous measurement of total enzyme amount and enzyme activity. Anal. Chem. 2011, 83, 3770-3777.
  8. Du, D.; Wang, L.; Shao, Y.; Wang, J.; Engelhard, H.M.; Lin, Y,H. Functionalized Graphene Oxide as a Nanocarrier in a Multienzyme Labeling Amplification Strategy for Ultrasensitive Electrochemical Immunoassay of Phosphorylated p53 (S392). Anal. Chem. 2011, 83, 746-752.
  9. Wang, Y. ; Li, Z.H. ; Wang, J.; Li, J. H.; Lin, Y.H. Graphene and graphene oxide: biofunctionalization and applications in biotechnology. Trends in Biotechnology. 2011, 29, 205-212.
  10. Wang, L.M.; Du, D.; Lu, D.L.; Lin, C.T.; Smith, J.D.; Timchalk, C.A.; Liu, F.Q.; Wang, J.*; Lin, Y.H. Enzyme-linked immunosorbent assay for detection of organophosphorylated butyrylcholinesterase: a biomarker of exposure to organophosphate agents. Anal. Chim. Acta, 2011, 693, 1-6 (Featured article).
  11. Fu, Z. F.; Shao, G. C.; Wang, J. * ; Lu, D. L.;  Wang, W. J.; Lin, Y. H. Microfabricated Renewable Beads-Trapping/Releasing Flow Cell for Rapid Antigen-Antibody Reaction in Chemiluminescent Immunoassay. Anal. Chem. 2011, 83, 2685-2690.
  12. Wang, L. M.; Lu, D. L.; Wang, J.; Du, D.; Zou, Z. X.; Smith, J.; Liu, F. Q.; Lin, Y. H. Biological monitoring trichloropyridinol, a biomarker of chlorpyrifos exposure, from the in vivo Rat plasma with a immunochromatographic electrochemical biosensor. Biosens. Bioelectron. 2011, 26, 2835-2840.
  13. Lu, D. L.; Shao, G. C.; Du, D.;  Wang, J.,  Wang, W. J.;  Lin, Y.H.   Enzyme entrapped nanoporous scaffolds formed through flow induced gelation in microfluidic filter device for sensitive biosensing of organophosphorus compounds. Lab Chip. 2011, 11, 381-384.
  14. Pinwattana, K. Wang, J., Lin, C.T., Wu, H., Du, D., Lin, Y.,  Chailapakul, O. CdSe/ZnS quantum dots based electrochemical immunoassay for the detection of phosphorylated bovine serum albumin. Biosens. Bioelectron. 201026, 1109-1113.
  15. Li, Z. H.; Wang, Y.; Wang, J.; Pounds, J.; Lin, Y. H. Rapid and Sensitive Detection of Protein Biomarkers Using a Portable Fluorescence Biosensor Based on Quantum Dots and a Lateral Flow Test Strip. Anal. Chem. 2010, 82, 7008-7014.
  16. Zhang, Y.Y.; Wang, J.; Tang, Z. W.; Wu, H.; Lin, Y.H. Hairpin DNA Switch for Ultrasensitively Spectrophotometric Detection of DNA Hybridization Based on Gold Nanoparticles and Enzyme Signal Amplification. Anal. Chem. 2010, 82, 6440-6442.
  17. Zhou, Z. X.; Du, D.; Wang, J.;* Smith, J. N.; Timchalk, C.; Li, Y. Q.; Lin, Y.H. “Quantum dot-based immuochromatographic fluorescent biosensor for biomonitoring trichloropyridinol, a biomarker of exposure to chlorpyrifos” Anal. Chem. 2010, 82, 5125-5133.
  18. Du, D.; Zou, Z. X.; Shin, Y.; Wang, J.; Engelhard, M. H.; Liu, J. Aksay, I. A.; Lin, Y.H. Sensitive Immunosensor for Cancer Biomarker Based on Dual Signal Amplification Strategy of Graphene Sheets and Multi-Enzyme Functionalized Carbon Nanospheres. Anal. Chem. 2010, 82, 2989-2995.
  19. Shao, Y.Y.; Wang, J.; Lin, Y.H. Graphene and its applications in sensors. Electroanalysis. 2010, 22, 1027-1036.
  20. Shao, Y.Y.; Wang, J.Engelhard, M.; Wang, C.M.; Lin, Y.H. Facile and controllable electrochemical reduction of graphene oxide and its applications. J. Mater. Chem. 2010, 20, 743-748.
  21. Kou, R.; Shao, Y.Y.; Wang, D.H.; Engelhard, M. H.; Kwak, J.H.; Wang, J.;  Viswanathan, V.V.; Wang, C.M.; Lin, Y.H.; Wang, Y.; Aksay, I. A.; Liu, J. Enhanced Activity and Stability of Pt catalysts on Functionalized Graphene Sheets for Electrocatalytic Oxygen Reduction. Electrochemistry Communications, 2009, 11, 954-957. 
  22. Smith, J.N.; Wang, J.; Lin, Y.H.; Timchalk, C. Pharmacokinetics of the Chlorpyrifos Metabolite 3,5,6-Trichloro-2-Pyridinol (TCPy) in Rat Saliva. Toxicolog. Sci. 2010, 113, 315–325.
  23. Du, D.; Wang, J.; Smith, J.N.; Timchalk, C.; Lin, Y.H. Biomonitoring of Organophosphorus Agent Exposure by Reactivation of Cholinesterase Enzyme Based on Carbon Nanotube-Enhanced Flow-Injection Amperometric Detection. Anal. Chem. 2009, 81, 9314-9320.
  24. Kang, X.H.; Wang, J.; Wu, H.; Aksay, I. A.; Liu, J.; Lin, Y.H. Direct electron transfer of glucose oxidase on a graphene-chitosan modified electrode. Biosens. Bioelectron.  2009, 25, 901-905.
  25. Ma-Ham, A.; Tang, Z.W.; Wu, H.; Wang, J.; Lin, Y.H. Protein-Based Nanomedicine Platforms for Drug Delivery. Small, 2009, 5, 1706-1721.
  26. Shao, Y.Y.; Kou, RWang, JWang, C.M.; Viswanathan, V.; Liu, J.; Wang, YLin, Y.H. The Durability Dependence of Pt/CNT Electrocatalysts on the Nanostructures of Carbon Nanotubes: Hollow- and Bamboo-CNTs. J. Nanosci. Nanotech. 2009, 9, 5811-5815.
  27. Wang, H; Wang, J; Choi, D; Tang, Z.W.; Wu, H; Lin, Y.H. EQCM immunoassay for phosphorylated acetylcholinesterase as a biomarker for organophosphate exposures based on selective zirconia adsorption and enzyme-catalytic precipitation. Biosens. Bioelectron.  2009, 24, 2377-2383.
  28. Barry, R. C.; Lin, Y.H.; Wang, J.; Liu, G.D.; Timchalk, C. Nanotechnology-based electrochemical sensors for biomonitoring chemical exposures. J. Exp. Sci. Environ. Epide. 2009, 19, 1-18.
  29. Lee, A. C.; Dai, Z.Y.; Chen, B.W.; Wu, H.; Wang, J.; Zhang, A.G.; Zhang, L.R.; Lim, T.M.; Lin, Y.H. Electrochemical Branched-DNA Assay for PCR-free Detection and Quantification of Oncogenes in Messenger RNA. Anal. Chem. 2008, 80, 9402-9410.
  30. Wang, H.; Wang, J.; Lin, Y.H. Magnetic-aided electrochemical immunoassay of phosphorylated acetylcholinesterase in plasma. Anal. Chem. 2008, 80, 8477-8484.
  31. Liu, G.D.; Wang, J.; Barry, R.; Timchalk, C.; Lin, Y.H. Nanaoparticle-based immunosensor for phosphorylated acetylcholinesterase, an biomarker for organophosphate pesticides. ChemEuro. J. 2008, 14, 9951-9959.
  32. Wu, H.; Huo, Q.S.; Varnum, S.; Wang, J.; Liu, G.D.; Nie, Z.M.; Liu, J.; Lin, Y.H. Dye-doped silica nanoparticle labels/protein microarray for detection of protein biomarkers. Analyst, 2008, 133, 1550-1555.
  33. Wang, J.; Lin, Y.H. Functionalized carbon nanotubes and nanofibers for biosensing applications. TRAC. Anal. Chem. 2008, 27, 619-626.
  34. Wu, H.; Engelhard, M. H.; Wang, J.; Fisher, D. R.; Lin, Y.H. Synthesis of lutetium phosphate-apoferritin core-shell nanoparticles for potential applications in radioimmunoimaging and radioimmunotherapy of cancers. J Mater. Chem. 2008, 18, 1779-1783.              
  35. Lin, Y.Y.; Wang, J.; Liu, G.D.; Lin, Y.H. Rapid and sensitive assay of prostate-specific antigen with immunochromatographic / electrochemical biosensor. Biosens. Bioelectron. 2008, 23, 1659-1665.
  36. Wang, J.; Timchalk, C. A.; Lin, Y.H. Carbon nanotube-based electrochemical sensor for assay of salivary Enzyme activity: an exposure biomarker of organophosphate pesticides and nerve agents. Environ. Sci. Technol. 2008, 42, 2688-2693.
  37. Shao, Y.Y.; Kou, R.; Wang, J.; Viswanathan, V. V.; Kwak, J. H.; Liu, J.; Wang, Y.; Lin, Y.H. The influence of the electrochemical stressing (potential step and potential-static holding) on the degradation of polymer electrolyte membrane fuel cell electrocatalysts. J. Power Source, 2008, 185, 280–286.
  38. Wang, J.; Liu, G.D.; Wu, H.; Lin Y.H. Electroactive Nanoparticle Label-Based Displacement Immunoassay of TNT. 2008, Anal. Chim. Acta, 2008, 610, 112-118.
  39. Wang, J.; Liu, G.D.; Wu, H.; Lin, Y.H. Quantum dot-based electrochemical immunosensor for high throughput screening of prostate-specific antigen. Small, 2008, 4, 82-86.
  40. Liu, G.D.; Lin, Y.Y.; Wang, J.; Wu, H.; Wai, C.M.; Lin, Y.H. Disposable electrochemical immunosensor diagnosis device based on nanoparticle probe and immunochromatographic strip. Anal. Chem., 2007, 79, 7644-7653.
  41. Wang, Y.J.; Karla, K. C.; Wang, J.; Alam, M. M.; Shyue, J. J.; Lu, W.X.; Padture, N. P.; Tseng, H. R. Individually addressable crystalline conducting polymer nanowires in microelectrode sensor array. Nanotechnology, 2007, 18, 1-6.
  42. Liu, G.D.; Wang, J.; Lin, Y.H. Versatile Apoferritin Nanoparticle Labels for Sensitive Assay of Protein. Anal. Chem., 2006, 78, 7417 - 7423.
  43. Liu, G.D.; Wang, J.; Wunschel, D. S.; Lin, Y.H. Electrochemical Proteolytic Beacon for Detection of Matrix Metalloproteinase Activities. J. Am. Chem. Soc., 2006, 128, 12382-12383.
  44. Wang, J.; Liu, G.D.; and Lin, Y.H. Sensitive Detection of a Biomarker TNF-a Based on Silica Nanoparticle Labels. Anal. Chem. 2006, 78, 6974-6979.
  45. Liu, G.D.; Wu, H.; Wang, J.; Lin, Y.H. Template Synthesis of Metal Phosphate Nanoparticle Labels for Electrochemical Immunoassay. Small, 2006, 2, 1139-1143.
  46. Wang, J.; Liu, G.D.; Lin, Y.H. Electroactive Silica Nanoparticles for Biological Labeling. Small, 2006, 2, 1134-1138.
  47. Liu, G.D.; Wang, J.; Lin, Y.H. Bioassay Labels Based on Apoferritin Nanovehicle. CHEMBIOCHEM, 2006, 7, 1315-1319.
  48. Wang, J.; Liu, G.D.; Lin, Y.H. Amperometric Choline Biosensor Fabricated through Electrostatic Assembly of Bienzyme/Polyelectrolyte Hybrid Layers on Carbon Nanotubes. Analyst, 2006, 131, 477-483.
  49. Wang, J.; Bunimovich, Y.; Sui, G.D.; Savvas, S. Y.; Heath, J. R.; Tseng, H.R.  Electrochemical Fabrication of Conducting Polymer Nanowires in an Integrated Microfluidic System. Chem. Commun. 2006, (29), 3075-3077
  50. Alam, M. M.; Wang, J.; Guo, Y.; Lee, S. P.; Tseng, H.R. Electrolyte-Gated Transistors Based on Conducting Polymer Nanowires. J. Phys. Chem.  2005, 109, 12777-12784.
  51. Wang, J.; Chen, S.; Carlson, R. R.; Luo, Y.; Ge, G.; Ries, R. S.; Heath, J. R.; Tseng, H.R. Electrochemically Fabricated Conducting Polymer Nanoframework-Electrode
  52. Wang, J.;* Grundler, P. Electrochemical studies of thiol self-assembled monolayers at a heated gold-wire microelectrode. Electroanal. 2003, 15, 1756-1761.
  53. Wang, J.;* Grundler, P. The influence of temperature on the interaction between DNA and metal complex at a heated gold-wire microelectrode. J. Electroanal. Chem. 2003, 540, 153-157
  54. Wang, J.; Li, J.H.; Baca, A. J. et al. Amplified Voltammetric detection of DNA Hybridization via Oxidation of Ferrocene Caps on Gold Nanoparticle/Streptavidin Conjugates. Anal. Chem.  2003, 75, 3941-3945.
  55. Wang, J.; Zhou, F.M. Scanning Electrochemical Microscopic Imaging of Surface- confined DNA probes and their hybridization via guanine oxidation. J. Electroanal. Chem. 2002, 537, 95-102.
  56. Wang, J.; Song, F.Y.; Zhou, F.M. Silver-Enhanced Imaging of DNA Hybridization at DNA Microarrays with Scanning Electrochemical Microscope. Langmuir, 2002, 6653-6658.
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